Inverter

Part:BBa_K377704

Designed by: Kevin Joseph   Group: iGEM10_Michigan   (2010-10-14)

FimB

FimB is a regulator of the fim operon, which produces Type I pili. FimB inverts an invertible DNA region containing the promoter for the fim operon, and has a slight preference for "on" [1]. It competes with FimE, which only inverts the region from "on" to "off".

Overexpression of this gene should lead to increased piliation, and we have used this part to attempt the induction of flocculation of E. coli with algae.


1. Schwan, W., Shibata, S., Aizawa, S., and Wolfe, A., The Two-Component Response Regulator RcsB Regulates Type 1 Piliation in Escherichia Coli Journal of Bacteriology 189 7159-7163 (2007)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None